Figure 1: Treatment of HeLa cells with IFN-alpha stimulates phopsphorylation of Stat3 at Tyr705 but does not affect the level of total Stat3 protein. OD450 readings are shown in the top figure, while the corresponding Western blot using anti-Phospho-Stat3 (Tyr705) or Stat3 rabbit monoclonal antibody , is shown in the bottom figure.
Figure 2: Linear relationship between protein concentration of lysates from IFN-alpha-treated HeLa cells and kit assay optical density readings. HeLa cells (75% confluence) were treated with IFN-alpha (100 ng/ml), and lysed after growth at 37oC for 10 min.
Supplier Page from United States Biological for STAT3, phosphorylated (Tyr705) ELISA Kit, BioAssay™ (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)